1439 Development of a complex skin organ in human induced pluripotent stem cell-derived organoids

A large amount of research has been devoted to developing skin organs for studying developmental pathways, modeling diseases, or regenerative medicine purposes. However, the field is still far from fulfilling its dream creating appendages, such as hair follicles and sweat glands. This challenge could be addressed with use human induced pluripotent stem cells (hiPSCs). Nevertheless, current hiPSC-derived substitutes lack nerves, fat which are critical function aesthetics. Here, we establish protocols engineering stratified layers their appendages using hiPSCs-derived organoids (SKOs) three different hiPSCs lines. The placodes start forming around Day 65 differentiation transform into by 83, expected during development. Further investigations confirm presence several stem/progenitor populations dermal papilla in mature SKOs 120. Additionally, can develop sebaceous glands, fat, touch-receptive Merkel cells. containing pigmented mimicking cellular composition architecture second-trimester fetal tissues successfully generated Together, hairy represent a step towards understanding follicle morphogenesis, enabling used reconstructive surgeries.